DNA-certification of breeds of bees of Ukraine in the system of preservation and development of their gene pool

Abstract

Goal. To develop a method of DNA passporting and to establish gene pool standards for purebredness in domestic beekeeping by molecular genetic fingerprint markers with multiple localization in the genome (RAPD, ISSR). Methods. Molecular genetic, statistical, morphometric, ethological. Results The results of molecular-genetic monitoring of bees of Ukrainian, Carpathian and gray mountain Caucasian breeds for using different technologies of detection of polymorphism of genetic loci are presented. Genetic passports of rocks were developed based on identified identification alleles. The absolute marker of the gray mountainous Caucasian breed G800, which was present in all its representatives, is a reliable tool for identifying families of indigenous origin of bees of Ukrainian aboriginal breeds. Conclusions Establishing genetically determined genetic differences, confirmed by genetic-population indicators, makes it possible to create natural reserves of pure-breed bees as a compulsory component in the system of preservation of their gene pool and breeding improvement.