Optimization of parameters of modification of surface of biosensor on the basis of ISUT for raising sensitivity at determination of Patulin

Abstract

Aim To determine the optimal parameters of determination to the patulin for sensitivity of the immune touch control on the basis of the ion-sensitive field transistors (ISFT). Methods. Biotechnological, biochemical, immunological. Results Both variants of "competition" of the method of analysis show that the amount of reduction in the signal in the range from 1 to 200 ng / ml-1 (in this range potential to the ISFT breech block of 9 to 98 mV). Some higher sensitivity and a range of measurable concentrations (0.5-250 ng / ml) have been extended a bit while it is discovered during the raising of the analysis of the method of "saturation". Conclusions. Establishment of optimal parameters of the work of this is an analytical tool for the use of different concentrations of H2O2, and the changes of pH showed him heterospecific reviews that substantially does not affect his work.