Molecular-genetic diagnostics of potato cyst-creating nematodes which are spread in zones of Polisya, Forest-Steppe and Ukrainian Carpathians

Abstract

Aim. To define specific composition of quarantine organisms – potato Cyst-forming eelworms, widespread in the zone of Polesye, Forest-steppe and Carpathians of Ukraine. Methods. Field (rout inspections), laboratory (extraction of eelworms is from the ground tests; authentication of eelworms is for the use of the multiplexed polymerase chainreaction with specific primersComplementary to the ribosomal gene 18S and internal Transcribed to the spacerof ITS1). Results. For the use of the multiplexed polymerase chain reaction (PCR) with specific primersdistribution of Globodera of rostochiensis is set within the limits of Ternopil (general area of the infected lands – 375,02 hectare), Zakarpattia (16,75 hectare) Chernivtsi (0,61 hectare) areas of Ukraine. A location of quarantine hearths in swingeing majority is on homestead lands of population. Conclusions. Expediency of further Validationmethod is witnessed for the use of more wide panel of standards of cyst-formingeelworms from the different regions of Ukraine. The got results are fixed in basis of recommendations in relation to realization of phytosanitarycontrol within the limits of the infected territories for effective localization and liquidation of found out quarantine hearths.