Detection of virus and bacterial contaminants of semen of pedigree bulls with the use of molecular-genetic methods

Abstract

The purpose. To tap contamination of deep-frozen semen of pedigree bulls with virus and bacterial agents. Methods. Bacteriological, molecular-genetic, statistical. Results. 826 samples from 6 areas of Ukraine are examined. That has allowed tapping genetic material of virus.es of contagious rhinotracheitis and diarrhea of cattle in 3,5 and 6, 1 % of events accordingly. DNA of Chlamydia and mycoplasma was taped in 3, 15 and 8,6% of the examined samples accordingly. The belonging of virus of the diarrhea found in semen of bulls to 2 genotype of the causal organism is fixed Conclusions. Considering existing risks of spread of communicable diseases through contaminated semen of bulls, there is a necessity of implementing measures on profound quality control of genetic resources of cattle.