Microclonal propagation black currant

Abstract

The purpose. To optimize separate stages of microclonal propagation black currant and to develop complex techniques of the sped up propagation for new cultivars on the basis of biotechnological methods. Methods. Preparation of nutrient mediums, and cultivation of plants in vitro was made under practical standards. Results. Specific features of deriving aseptic crop black currant of cultivar Saniuta and elite form 01 - 1 - 9 are probed. Nutrient mediums for proliferation and rootage in crop in vitro and methods of acclimatization to conditions ex vitro are selected. Positive influence of Acidum ascorbinicum upon efficiency of cultivation of microscions is shown. Conclusions. For deriving aseptic crop black currant as explants the most suitable are apical and lateral buds got from green scions. Addition of Acidum ascorbinicum (0,57 mM) into medium for initiation of aseptic crop and proliferation prevents depression of recovery and growth activity of explants which is caused by oxidation of phenols. Adaptation on substrate «Domoflor mix 3» ensures neogenesis 91 – 98% of microscions.