Features of karyotyping variability of daughters of bulls-embrio-grafts

Abstract

The purpose. To investigate cytogenetic characteristicsof cows which descend from the bulls gained by themethod of embrio-transplantation and cows-descendantsof bulls, born after artificial fertilization. Methods.Cultivation of lymphocytes, preparation of cytogeneticspecimens, classification and account of aberrationsof chromosomes realized under practical standards.Results. Abnormalities of karyotype of genome type(aneuploidy and polyploidy) and structural aberrationsof chromosomes (fragments, breakages, shortages andassociations of chromosomes) are determined by resultsof analysis. General frequency of karyotypic infringementsat cows-daughters of bulls from embryo-transplantationwas higher for 10% in comparison with indexat cows-daughters of bulls from artificial fertilization.At cows that had even one deadborn or spontaneousabortions, frequency of chromosomal infringements wasconsiderably higher, than at animals without coarse reproductiveinfringements. Conclusions. For preventingaccumulation of genetic imperfections in herds of cattleit is necessary to spend regular cytogenetic monitoringof animals.