Virus diseases of plants in agrocenosis and forest ecosystems: diagnostics and prevention

Abstract

Goal. To analyze the already known results of virological tests and to investigate virus diseases in agricultural lands and forest ecosystems. Methods. Visual examination, enzyme-linked immunosorbent assay in various modifications, allocation of total RNA from plant material, purification of cDNA, electrophoresis of nucleic acids, CT-PCR, and me­thod of statistical data processing. Results. They analyzed the results of domestic and foreign researches on phytovirusology. Joint research was conducted in the Institute of Agroecology and environmental management of NAAS and the National University of life and environmental Sciences of Ukraine regarding the prevention of viral infections of plants at the use of the composition of basidiomycetes fungi. For the recovery of plants from viral diseases, they used a process of simulated microgravity. Conclusions. Taking into account scientific, environmental and social importance of biological security in agriculture as an important condition of balanced development of Ukraine and the production of quality food products, agriculture and forestry should be based primarily on the use of quality certified disease-free seed of resistant varieties. So pressing is the problem of the creation of the Depositary of the main viruses affecting agricultural and forest crops, and scientific center «Virus», which was initiated by the Bureau of the Presidium of the National Academy of agrarian sciences of Ukraine on 18 September 2019.