The efficiency of modern disinfectants and detergents for sanitization of dairy equipment

Abstract

Goal. To determine the efficiency of modern disinfectants and detergents for the sanitization of dairy equipment. Methods. Study of the sensitivity of planktonic and biofilm forms of microorganisms to disinfectants was carried out on 18–24-hours cultures, isolated from dairy equipment by the methodology of M. D. Kuhtin et al. Results. It is determined that working solutions of disinfectants P3-oxonia active-150, Eco chlor, Ultra circ, Maxidez showed bactericidal activity against planktonic bacteria. However, the bacteria formed in the biofilm had increased stability to these solutions of disinfectants. The most effective means for the destruction of the microbial biofilms was the preparation P3oxonia active-150 based on hydrogen peroxide and peracetic acid. It showed a bactericidal effect on biofilm of bacteria Streptococcus spp., Staphylococcus aureus, Enterococcus faecalis, and Pseudomonas fluorescens. The preparation reduced the number of bacteria in biofilms formed by Escherichia coli, Lactobacillus spp., Bacillus spp., and P. aeruginosa, up to 500 CFU/cm3 of wash off. Pseudomonas aeruginosa was the most resistant to disinfectants. Only planktonic forms of P. aeruginosa were sensitive to Ultra circ and Maxidez, and biofilm forms were resistant to all preparations in the experiment. Conclusions. Argenvit was ineffective against biofilm and planktonic forms of bacteria. Disinfectants P3-ansep CIP, Eco chlor, Ultra circ, and Maxidez had a bactericidal effect on planktonic bacteria but did not affect biofilm forms. The most effective means against the bacteria in biofilms was P3-oxonia active-150 based on hydrogen peroxide and peracetic acid. Therefore, for effective sanitization of dairy equipment is necessary to use such disinfectants that affect the bacteria in biofilms.