Creation of information base of boars of Myrgorod breed, which cryopreserved sperm is stored in the bank of animal genetic resources

Abstract

Goal. To provide information about boars of Myrgorod breed, which sperm production is stored in deep-chilled state in the Bank of animal genetic resources (Institute of genetics and animal breeding named after M.V. Zubets of NAAS) in the context of the formation of the structured information on animal genetic resources at the national level and possible recovery of the breed using modern methods. Methods. The study was conducted on boars of Myrgorod breed belonging to the lines Veselyi, Dnipro, Komysh, and Lovchyk. The study of immunogenetic characteristics of boars was carried out by 9 genetic systems of blood groups: EAA, EAB, EAD, EAE, EAF, EAG, EAH, EAK, and EAL. The processing involved data from all boars of these lines that were in the breed. Assessment of boar semen was carried out on the ejaculate volume and mobility of sperm cells in the laboratory of artificial insemination of breeding plant SE «SF named after Decembrists of the Institute of pig breeding and agro-industrial production of NAAS». They took semen from boars during the year in the mode of the sexual use — one cage in 3 days. They determined the average volume of ejaculate and motility of sperm cells. The characteristic of boars as to the productivity was made on the indicators of live weight, body length, age of reaching a live weight of 100 kg during growth, and multiple pregnancies in sows inseminated by them. Results. It was found that among the examined boars the most common allelic variants were A, Ba, Db, Eedg, Eaeg, Fb, Gb, H, Kb, Lb with a frequency of 0.233–1.000. To the category of relatively rare were read alleles Eaef, Ka, Ld with a frequency of 0.033–0.067. The boars of Myrgorod breed did not differ significantly among themselves for the most of the genetic systems of blood groups, besides systems of EAE, EAK. and EAL. The obtained results give the possibility to realize a homogeneous or heterogeneous selection and to create desirable genotypes in the breed in case it is subjected to recovery. The largest volume of ejaculate sperm was obtained from boars of lines Veselyi and Dnipro (223.8 and 227.4 ml respectively), the smallest – from line Komysh (139.6 ml), which was probably due to the individual characteristics of animals and their heredity. The sperm of the studied boars were not significantly different in the mobility of sperm cells (9.0–9.5 points). Conclusions. The characteristics of the boars-manufacturers on the genetic systems of blood groups, sperm quality, and performance are given. This information can be used to retrieve ancestors of lines or families in the recovery process of the Myrgorod breed of pigs and the creation of other genotypes. It will also help to solve the complex problems of preservation and use of local endangered breeds of agricultural animals of Ukraine under the recommendations of the Commission on animal genetic resources of FAO.