Determination of genetically modified rape and monitoring of its spread

Abstract

The purpose. To complete methodical marching and to develop diagnostic test-system on the basis of method of polymerase chain reaction (PCR) in real time for determination of genetically modified rape (GM). Monitoring of spread of biotechnological rape in Ukraine. Methods. Molecular-genetic — extraction of DNA, PCR; biotechnological — development of test-system on the basis of PCR-method in real time (Real-Time PCR). Results. Screening testsystem is developed for determination of GM rape by PCR-method in real time. The system allows to analyze both on regulatory elements (tNOS), and on structural genes (CP4 epsps, Pat, Bar, bay TE, bxn). By means of the developed system monitoring of spread of biotechnological rape in Ukraine is carried out, and availability of Roundup Ready™ rape in amounts exceeding 0,9% is shown. Conclusions. The gained results testify to necessity of supervisory control of agricultural raw, in particular seeds of rape, for the content of genetically modified organisms (GMO).