Improvement of diagnostic test-system of the Teshen illness on the basis of solid-phase immunoenzymatic analysis

Abstract

The purpose. To improve immunoenzymatic test-system for detection of immunoglobulins of class G to a virus of Teshen illness in blood serum of pigs. Methods. Virologic, serologic, immunoenzymatic, instrumental and statistical methods offered by foreign and domestic scientists, as well as own ones. Results. At use of new industrial strain Porcine teschovirus of the first serotype Dneprovsiy-34 they improved diagnostics of Teshen illness of pigs on the basis of solidphase immunoenzymatic analysis. Conclusions. Specificity of the test-system makes 100%, responsivity — 95,6 %. Application of the test-system allows making the diagnosis during 7 hours, that it is much less in comparison with reaction of neutralization.