Physiological-biochemical responses in seeds and plants of pease (Pisum sativum L.) at initial stages of ontogenesis at action of biological products and growth regulators of plants.

Abstract

The purpose. To probe physiological-biochemical responses in seeds, roots and shoots of pease at initial stages of ontogenesis at use of biological products and growth regulators of plants. Methods. Seeds of pease (Pisum sativum L.) of grade Glians were treated with working solutions of growth regulator of plants AKM and microbial specimen Rizobofit. Seeds were germinated in containers with sand in a thermostat at temperature of 20±2°C. Intensity of peroxide oxidation of lipids in tissues of germinating seeds, young plantlets and roots was evaluated according to the content of malonic dial (MD) which was determined with the help of spectrophotometric method and recalculation for dry matter (DM). That index, and also mass of dry matters was determined at different stages of growth of pease BBCH (00, 03, 05, 08, 12, 13, 14, 15) according to practical standards. Results. It is determined that presowing treatment of seeds with growth regulator of plants AKM and its mixture with microbial specimen Rizobofit during heterotrophic nutrition makes active metabolic processes in mericarp, stimulates germination, increases dry mass of roots by 23 and 37% and decreases intensity of POL processes by 37,5 and 24% in comparison to the control. With transfer to autotrophic type of feed the dry mass of seed-lobe intensely drops at treating with AKM and its mixture with Rizobofit. That process is accompanied by activation of growth processes in roots and plantlets, and increase in their mass. POL intensity in roots drops, that testifies to formation of adaptive response to physiological and chemical stresses at germination and formation of nodules. Conclusions. It is proved that these specimens manifest phyto-stimulating and adaptogenic effect on the processes of germination of seeds and initial propagation of roots and shoots of pease (Pisum sativum L.).