Use of preparation Lizoformin 3000 for obtaining aseptic cultures of honeysuckle in conditions in vitro

Abstract

The purpose. To investigate influence of preparation Lizoformin 3000 against pathogenic microflora at obtaining aseptic cultures of honeysuckle (Lonicera edulis Turcz) and on the further regeneration of plants depending on method of sterilization and state of initial plants. Methods. Laboratory, mathematical, calculation-comparative. Results. Influence of sterilizing substances on obtaining aseptic cultures of honeysuckle in conditions in vitro is studied. Experiment were spent on grades: Alisia, Spokusa, Chaika, Nimfa, Dochka Veletnia, Karina. As sterilizing agents they used Lizoformin 3000 at different time of sterilization and solution of mercury bichloride as the control. Stages of sterilization on the control included the following components: 1) treatment of explants in solution of sodium hipochlorite — 20 min with subsequent washing in water; 2) sterilization with spirit (C2H5OH) — 20 sec with washing in water; 3) sterilization in solution of mercury bichloride (HgCl2) — 2 min with 3-times washing in sterile distilled water. In the variant with studying preparation Lizoformin 3000 instead of solution of mercury bichloride they used the studied preparation at expositions of 5, 7 and 10 min. Conclusions. For the maximal obtaining of sterile and viable explants the sterilizing agent and its toxicity has great value. Preparation Lizoformin 3000 at corresponding concentration and duration of sterilization should be recommended for obtaining aseptic cultures of honeysuckle. On the background of application of that preparation with exposition of 5 min they allocated by efficiency of regeneration 3 groups of varieties of honeysuckle: with high reclaiming ability (94 – 96%) — Alisia, Karina and Spokusa; with average reclaiming ability (86 – 87%) — Chaika and Dochka Veletnia, and with low reclaiming ability (80%) — Nimfa. Preparation Lizoformin 3000 in concentration of 3% and with duration of exposition of 5 min provides optimum efficiency of sterilization and regeneration of explants of honeysuckles and does not reduce their factors of duplication. Preparation Lizoformin 3000 at corresponding concentration and duration of sterilization should be recommended for obtaining aseptic cultures of varieties of honeysuckle.