Lectin activity of winter wheat seedlings affected by eyespot causal agent under the action of Bacillus subtilis 537/Б1 bacterial isolates

Abstract

The purpose. To study effect of bacterial isolates Bacillus subtilis 537/B1 upon change in lectin activity and content of malonic dialdehyde (MDC) in plantlets of winter wheat of different on resistance varieties — Myronivska 808 and Renan — affected by causal organism of eyespot spot Pseudocercosporella herpotrichoides (Fron) Deighton. Methods. Preparation of lectin-containing extract was carried out by Lutsyk method. Lectin activity was determined by erythro-agglutination method. Protein content in lectin-containing extracts was determined by Bradford method. Level of generated MDC as a product of lipid peroxidation was estimated according to Dhindsa and Matowe. Statistical analysis of gained results was made by method of dispersion analysis in the program Microsoft Excel. Reliability of variance between alternatives was evaluated by Student t-test number at P≤0,05. Results. It is shown that treatment of plantlets of winter wheat of varieties Myronivska 808 and Renan wth bacterial isolates of B. subtilis 537/B1 in pathogeny conditions promoted the maximum growth of lectin activity. And for variety Myronivska 808 such treatment was more efficient. It was established that oxidative stress developed both in sprouts and seeds infected with conidia suspension of pathogenic fungi and suspension of B.subtilis 537/B1 bacterial isolates. That was confirmed by intensification of lipid peroxidation (LPO) processes. Conclusions. Treatment of plantlets and seeds of winter wheat of varieties Myronivska 808 and Renan with bacterial isolates B. subtilis 537/B1 had protecting character under the action of eyespot causal agent. The obtained data may become a basis for the further researches and consideration of opportunities for creation of new efficient biofungicides on the basis of B. subtilis 537/B1 for protection cereal crops against causal organisms of different diseases.