Development of molecular-biological system to identify RNA-4 of Ukrainian isolate of the virus of necrotizing yellowing of veins of beet

Abstract

Goal. To develop a system for the identification of RNA-4 Ukrainian isolates of the necrotic yellowing virus of beetroot veins (VNPZHB) on the basis of the polymerase chain reaction. Methods. Molecular- biological. Results A bioinformative analysis of nucleotide sequences of RNK-4 isolates of VNPZHB was carried out. Conservative sequences of the gene encoding protein R31 VNPZHB were established, and primer design was developed for the identification of RNA-4. The PLR-system of identification of RNA-4 has been optimized for the temperature indices of the annealing of primers. Conclusions The developed diagnostic system allows timely identification of VNPZHB to prevent its spread.