The karyotype of ukrainian mountain carpathian sheep breed

Abstract

The purpose. To study cytogenetic features of Ukrainian mountain carpathian sheep breed. Methods. Karyotype of 25 sheep of ukrainian mountain carpathian sheep breed of FF “Radvan-Nova” (Myloshevychi, Pustomytiv region, Lviv oblast) was probed by microscopic research of specimens of cells at the stage of metaphase of mitosis got from short-term culture of peripheral blood lymphocytes of animals. Preparation of specimens of chromosomes, analysis of morphology, grading and record-keeping aberrations of chromosomes was realized on the conventional procedures in laboratory of genetics of Institute of animal growing and genetics. Results. It was fixed that amount and structure of chromosomes of ukrainian mountain carpathian sheep breed matched to the norm, characteristic for that kind of animals. In structure of karyotype were acrocentric and metacentric chromosomes. From 722 analysed metaphase plates of sheep the share of aberrant cells made 19,22 %, of them aneuploid cells — 8,6, polyploid — 0,86, cells with breakages of chromosomes — 2,22, frequency of paired fragments of chromosomes — 2,66 and frequency of cells with asynchronous divergence of centromeric regions of chromosomes — 4,88 %. Conclusions. By number and structure of chromosomal complement ukrainian mountain carpathian sheep do not differ from other breeds of sheep. Of 27 pairs of chromosomes of karyotype of sheep chromosomes of I group of metacentric structure (which are bigger than other chromosomes) and gonosomes are identified with adequate accuracy. In connection with such feature it is possible to determine with what frequency these chromosomes participate in structural aberrations. Identification of separate chromosomes at routine color enables to use cytogenetic probes in practice of selection operation in sheep-breeding.