Detection of virus, bacterial and phytoplasmic diseases on vineyards of Odesa oblast

Abstract

The purpose. To determine presence of virus, bacterial and phytoplasmic diseases of grape on grape plantings of Odesa oblast. Methods. Field, laboratory. Fytosanitory diagnostic study of vineyards in grape-cultivation farms (Ovidiopol and Tatarbunar regions of Odesa oblast). They identified causal organisms of diseases of grape plants using serological (immunoenzymatic — ELISA) and molecular-biological (polymerase chain reaction (PCR) with electrophoretic detection) methods. Results. At visual diagnostic study of grape plantings in Ovidiopol region they detected plants with symptoms of leaf curl and on occasion — with symptoms similar to that caused by fanleaf virus and virus of a complex striation of wood of a grape. Grape plants with symptoms of bacterial cancer and phytoplasmic diseases are detected on vineyards of Ovidiopol and Tatarbunar regions. Presence of causal organisms of virus (fanleaf of grapevine, leaf curls, and viruses of a complex striation of wood of a grape), phytoplasmic diseases and bacterial cancer is confirmed by methods of ELISA and PCR. High scale of defeat is registered by the causal organism of bacterial cancer on scrubs of variety Cabernet Sovinion, by the causal organism of phytoplasmic disease of wood blackening — on variety Shardone. Conclusions. The greatest percent of plants of grape struck by virus and phytoplasmic diseases, has appeared in Ovidiopol region, by the causal organism of bacterial cancer — in Ovidiopol and Tatarbunar regions of Odesa oblast. It is established that approximately 10% of the determined diseases of grape are virus ones, nearby 25 are bacterial cancer, and 65% are phytoplasmic diseases.