Analysis of oocyte-cumulus complexes of goats and parameters of their maturation in conditions in vitro

Abstract

Purpose. Analysis of populations of oocyte-cumulus complexes of goats and determination of optimal time parameters of their maturation under in vitro conditions. Methods. Genetic (cytogenetic analysis of chromosomal changes during maturation of oocytes in vitro), biometric. Results It was established that by cytomorphological features of oocyte-cumulus complexes of goats, suitable for further in vitro cultivation, ovaries at the stage of follicular growth were obtained by 15.9% more than in the luteal stage. Conclusions To obtain oocyte-cumulus complexes of goats it is expedient to use ovaries at the stage of follicular growth. Optimal time of cultivation of oocytes of goats in vitro for reaching stage II meioze metaphase - 27 hours.