Assessment of collection samples and kinds of winter rape as to resistance to low temperatures at the level of microgametophyte

Abstract

The purpose. Assessment of cold resistance of selection material by man gametogophyte, determination of optimum reduced temperature regimen for sprouting blossom dust, selection of cold- resistant genotypes with their further use in selection process for creation on their base of kinds and hybrids with high ecological toughness. Methods. Method of sprouting of blossom dust in nutrient medium in conditions of action of the factor. Cold resistance was assessed by ability of blossom dust to germinate and form long pollen tubes. Results. Sprouting of blossom dust in conditions of cold caused decrease of percentage of sprouting and length of pollen tubes in experimental alternatives. Temperature regimens 2±1 and 3±1°C have greater selective action in comparison with temperature regimens 5±1 and 7±1°C and can be offered as optimum for cold sprouting of blossom dust. Conclusions. It is determined that cold sprouting of blossom dust in nutrient medium in greater extent influenced percentage of germination of blossom dust, than length of pollen tubes. Average extent of lowering by attributes has made accordingly 72,7 and 41,5%. For the further selection researches 3 kinds which have manifested durability to cold to both attributes are offered.