Cultivation of plants of potato in vitro at microclonal reproduction

Abstract

The purpose. To determine optimum root formation in crop in vitro of medium-early cultivars of potato Nevskaya depending on acidity of nutrient medium(pH), intensity of illumination and photoperiod for increaseof production of the initial improved planting stock. Methods. Complex use of laboratory, mathematical and statistical, calculation-comparative methods and system analysis. Results. Experimental data concerning influence of acidity of nutrient medium (pH), intensity of illumination and photoperiod on induction of root formation are brought at microclonal reproduction of the improved initial material. It is fixed that pH of nutrient medium influences intensity of root formation. Conclusions. The maximum productivity indexes and economic efficiency of plants in vitro of medium-early cultivar Nevskaya are gained at pH=5,3, photoperiod of 16 h and illumination of 2500 l. Intensity of root formation made 92,7%, mass of average microtuber — 667,7 mg, mass of microtubers for 1 plant — 617,3 mg, amount of microtubers in mass more than 350 mg — 79,1% at profitableness of production of 117 %, cost prices 7,37 hrn/microtuber, conditional net profit — 8,63 hrn/microtuber.