Efficiency of use of light-emitting diodes for clonal microreproduction of sweet potato and peppermint in crop in vitro

Abstract

The purpose. To determine efficiency of application of state-of-the-art LED-systems for clonal microreproduction in crop in vitro of individually selected, with high biochemical parameters of clones of sweet potato and peppermint. Methods. Methods of cultures of organs and tissues — culture of somatic tissues and cells, clonal microreproduction, measuring-weight, mathematical-statistical. Results. At use of light-emitting diode lamps of red and dark blue colors in tubed plants they observed increase of average length of shoot: for peppermint — on 31,2%, for sweet potato — on 10,6 % in comparison with control alternative (cultivation under luminescent lamps). The maximum multiplication ratio of peppermint has provided genotype M.r-17/4 — 7,5±0,6 pieces (control) and 7,9±0,6 pieces — cultivation under LED-lamps. At plants of sweet potato cultivated under light-emitting diode lamps owing to augmentation of length of internodes from 5,2 mm (control) to 6,7 mm the multiplication ratio has increased from 5,5 (control) to 5,8 pieces. Application of light-emitting diode lamps of red and dark blue colors for tubed clones of peppermint and sweet potato has provided tall multiplication ratio of perspective for selection and seeds growing of genotypes of well crops. Spectral make-up of light-emitting diodes according to biological features of crop and stage of morphogenesis requires the further optimization. Conclusions. Use of light-emitting diode lamps of red and dark blue colors placed on light-emitting diode tape in combination 2 1, provides necessary illuminance for growth of plants of tubed clones of peppermint and sweet potato at the stage of their mass reproduction and reduces expenses for the electric power for 62,5 %.