Activity of sucrose phosphate synthase in lines of sugar beet (Beta vulgaris L.) with complex stability

Abstract

The purpose. To determine activity of enzyme sucrose phosphate synthase in lines of sugar beet with complex stability to two stressful factors. Methods. Laboratory, vegetative, mathematical-statistical. Results. Processes of metabolism break during adaptation of plants to stressful factors. That is reflected in oppression of growth and decrease in photosynthetic activity. Experimental data on change of functional activity of enzyme sucrose phosphate synthase in conditions of action of water and salt stresses in leaves of plants-regenerants and sugariness of root crops of cellular lines of sugar beet with complex stability to drought and salinization are brought. At experiment the generated plants-regenerants of cellular lines of sugar beet of height 10–15 cm which had 6–10 true leaves were replanted in the open ground. Their survival made 68-80%. The revealed essential changes of functional activity of enzyme sucrose phosphate synthase in researched regenerants of cellular lines at influence of complex stress found confirmation in formation of sugariness of root crops. Thus especially perceptual was activation of enzyme in highly-stable cellular lines which size varied within the limits of 60-99%. That testified to their ability to adaptation in such conditions. It was established that sugariness of the received lines of sugar beet with complex stability against drought and salinization depending on their gradation was within the limits of 15,8–17,2% and decreased under these conditions concerning control plants on 0,2–0,7%. Conclusions. At complex stress there are essential changes in action of enzyme sucrose phosphate synthase in leaves and sugariness of root crops. Activity of enzyme can be used as biochemical marker of stability in conditions of primary diagnostics of plants-regenerants of sugar beet with potentially high adaptive potential.