Distribution of mitochondrial haplotypes in groups of pigs of Myrhorod and Poltava meat breeds

Abstract

Goal. To find out how mitochondrial haplotypes are distributed in groups of Myrhorod and Poltava meat breeds of pigs, to use sows of the Poltava meat breed to restore the population of the Myrgorod breed of pigs. Methods. DNA typing of samples of Myrhorod and Poltava meat breeds of pigs was carried out (20 animals of each breed). DNA was isolated using Chelex-100 reagent. Animal genotyping for mitochondrial haplotypes was performed using polymerase chain reaction techniques followed by restriction fragment length analysis. Results. It was found that the total share of typical mitochondrial haplotypes in the samples of pigs of Myrhorod breed was 80% (haplotypes B1, C/B2, L), and in the samples of Poltava meat breed — 90% (haplotypes B1, J1, O, C), 10% of which falls were of the haplotype J1, which was inherent in a Large white breed of pigs. Genotyping showed that the number of animals of the Poltava meat breed with the desired mitochondrial haplotypes was sufficient to provide the possibility of selecting and assorting pigs for this molecular marker for crossing sows of the Poltava meat breed with boars of the Myrhorod breed. Conclusions. Genotyping of samples of pigs of Poltava meat and Myrhorod breeds belonging to the experimental base of the Institute of Pig Breeding and Agro-Industrial Production of NAAS indicated the presence of pigs with mitochondrial haplotypes, inherent in the Myrhorod breed, in the Poltava meat breed of pigs. The proportion of typical mitochondrial haplotypes in the sample of pigs of Myrhorod breed from the livestock of 2024 was also obtained. — it was 80%. Consequently, the approach to the selection of pigs according to the mitochondrial haplotypes of the Poltava meat breed can be applied to restore the population of the Myrhorod pig breed.